| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090948 |
| Chromosome: | chromosome 16 |
| Location: | 4802847 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686350 | (1 of 1) IPR000738//IPR009068 - WHEP-TRS domain // S15/NS1, RNA-binding | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCACCACGCACCCACGGGACACGACGG |
| Internal bar code: | CGGTAAATATCCCCTATGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 98.7465 |
| LEAP-Seq n confirming: | 2442 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGCTGTTCTACCAAGGG |
| Suggested primer 2: | TCTAACACAGCTTGCCGATG |