| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090963 |
| Chromosome: | chromosome 12 |
| Location: | 4480874 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521650 | (1 of 3) PTHR22753:SF1 - TRANSMEMBRANE PROTEIN 68 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGGTCACCCGGCCAACGACCACCCGT |
| Internal bar code: | CCGGGGCGAATAGTCTTACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 571 |
| LEAP-Seq percent confirming: | 99.5864 |
| LEAP-Seq n confirming: | 6261 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAAGCAACTAAGGCAGG |
| Suggested primer 2: | AAGGTGTAAGCTTGTGCGCT |