| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090980 |
| Chromosome: | chromosome 4 |
| Location: | 2417165 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221301 | CYCD5 | D-type cyclin; (1 of 10) PTHR10177 - CYCLINE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATTGCGTCATCCGGTAACCCTGCCACT |
| Internal bar code: | GGGCGTGGCGTGTCCCGGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 30 |
| LEAP-Seq percent confirming: | 97.732 |
| LEAP-Seq n confirming: | 474 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTTCGCCTTCCTACTCT |
| Suggested primer 2: | CTAGTTTGCCACCGAAGCTC |