| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090983 |
| Chromosome: | chromosome 11 |
| Location: | 3647218 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482300 | FAP305,MOT17 | Motility 17; (1 of 1) PTHR22706:SF0 - SPERMATOGENESIS-ASSOCIATED PROTEIN 17 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCAGGTGCGTGGGCGCATGCAAGTGCAG |
| Internal bar code: | GGAAGTGATACGCGTACTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 99.5958 |
| LEAP-Seq n confirming: | 2464 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGACGAACAGAGTGGC |
| Suggested primer 2: | CGTGGTACAATCAGGTGTGC |