Insertion junction: LMJ.RY0402.090996_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.090996
Chromosome:chromosome 9
Location:4746196
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g397179 OPR13 OctotricoPeptide Repeat protein 13 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCCAATCCCACACCAGCCCTCACGCCA
Internal bar code:GCGGCGTAGTGGACTTGACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:433
LEAP-Seq percent confirming:98.3661
LEAP-Seq n confirming:6502
LEAP-Seq n nonconfirming:108
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CACACAGCAGCTTCAACCAT
Suggested primer 2:GCAGTCACGGACCAATACCT