| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090999 |
| Chromosome: | chromosome 2 |
| Location: | 2784711 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094150 | HIK,HIK2,HKR2 | (1 of 1) K12130 - pseudo-response regulator 5 (PRR5); Response regulator of two-component system | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCTTCACACTCCACTCCCTGTCTTCAA |
| Internal bar code: | TCGAAGAATAGGGGTCCCCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 99.0926 |
| LEAP-Seq n confirming: | 1638 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATACCCTTCCCACCTCCAT |
| Suggested primer 2: | TGTTGGCAGAGAGAGTGGTG |