| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091011 |
| Chromosome: | chromosome 9 |
| Location: | 5679547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401367 | (1 of 1) PTHR15189:SF7 - BRCA1-A COMPLEX SUBUNIT BRE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAAAGAGGTGTGTGGGTCTGGCGCTGC |
| Internal bar code: | TATGAGTGAACATCCCGACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 539 |
| LEAP-Seq percent confirming: | 99.5726 |
| LEAP-Seq n confirming: | 466 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCTCATGAATACAGCCC |
| Suggested primer 2: | TTCAAATGGAGTTGCAGACG |