| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091014 |
| Chromosome: | chromosome_1 |
| Location: | 5199941 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g036200 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTGGCATGGTGTGGTGTCGCATGGTGCGG |
| Internal bar code: | TAGATTCCACACGACCGCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 66.5644 |
| LEAP-Seq n confirming: | 1085 |
| LEAP-Seq n nonconfirming: | 545 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATCGAGGTGGAGGTTGT |
| Suggested primer 2: | CACGCCGAGTGTAGTCAGAA |