| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091041 |
| Chromosome: | chromosome 13 |
| Location: | 1347897 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g571500 | CST2 | Chlamydomonas-specific membrane transporter of unknown function | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGCCATTCAAGCGTTGGCCACCTCTCCG |
| Internal bar code: | GAAACTGGCCGTTGACGCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 99.5389 |
| LEAP-Seq n confirming: | 3022 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGTGCTGAAATATGCTGAA |
| Suggested primer 2: | ACTGGTTCATCACCTCCCTG |