| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091048 |
| Chromosome: | chromosome 5 |
| Location: | 2909225 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238100 | PTK25 | (1 of 66) 2.7.12.1 - Dual-specificity kinase; Protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTCCCATGAGCATCTAAATCACCCGCA |
| Internal bar code: | TACCCTTCTTGCTAGAAGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 99.6517 |
| LEAP-Seq n confirming: | 4006 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGCCCATCAAGTACACC |
| Suggested primer 2: | TAAACAACCACACCGCACAT |