| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091060 |
| Chromosome: | chromosome 17 |
| Location: | 276676 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698200 | MMS21 | SUMO ligase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGACTACCTGACGGTGCTAGGAGACACG |
| Internal bar code: | AAGACAGCGCCCTTCTTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 79.1398 |
| LEAP-Seq n confirming: | 368 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGCGAGGTGCAGCATA |
| Suggested primer 2: | CTCGTCCTCCTCCTCTTCCT |