| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091079 |
| Chromosome: | chromosome 1 |
| Location: | 6917614 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g049826 | PHC59 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACTGGTGCTCCTAACCTGCAATCTCCC |
| Internal bar code: | CCAGGCGCGATGTACCGCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 243 |
| LEAP-Seq percent confirming: | 99.6415 |
| LEAP-Seq n confirming: | 12509 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTGAGTAGGCGATGTCA |
| Suggested primer 2: | TATGTTTGACTGGAGCTGCG |