| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091102 |
| Chromosome: | chromosome 10 |
| Location: | 739578 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422750 | MOT10 | Predicted protein; (1 of 3) IPR002110//IPR005821//IPR020683 - Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAGCCCGCCGCCCCGCGCTCCATCTGCT |
| Internal bar code: | CACATTTGGGTGTAGTCAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 307 |
| LEAP-Seq percent confirming: | 99.7154 |
| LEAP-Seq n confirming: | 7709 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCTGGCACGCATCTACA |
| Suggested primer 2: | ATCGTTCGACATGTTCCCTC |