Insertion junction: LMJ.RY0402.091104_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.091104
Chromosome:chromosome 3
Location:6743538
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g198000 PPP15 (1 of 2) PF00481//PF13672 - Protein phosphatase 2C (PP2C) // Protein phosphatase 2C (PP2C_2); Phosphoprotein phosphatase 2C-related 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCGCGGCGTCTACAGAGCGTCTATGGC
Internal bar code:TGCGGCGCCGTGTCCTGACTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:649
LEAP-Seq percent confirming:99.1964
LEAP-Seq n confirming:3703
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTCAAGAGGCTTCGGTC
Suggested primer 2:ACTCCTGGCAGTTATGGGTG