Insertion junction: LMJ.RY0402.091124_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.091124
Chromosome:chromosome 7
Location:788985
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g318050 (1 of 10) PF00170 - bZIP transcription factor (bZIP_1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACCGGCGACGCCTTTGCGATTAGCTGAA
Internal bar code:CCGGGATCGCGTTGGGGCGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:348
LEAP-Seq percent confirming:91.073
LEAP-Seq n confirming:8080
LEAP-Seq n nonconfirming:792
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCTACTGTGCTCGACTTC
Suggested primer 2:GTCACGACCATTCCGTCTTT