| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091146 |
| Chromosome: | chromosome 6 |
| Location: | 6340236 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291950 | MRS4,MRS3 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGCAGCCAGCTGCGAACGCTGTAGCCG |
| Internal bar code: | CTAGCGTAGATGTTTACCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 83.7838 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTCTTTGCACCCTCTG |
| Suggested primer 2: | TATGTCCAGCAGCCTCACAG |