| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091146 |
| Chromosome: | chromosome 12 |
| Location: | 657066 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492050 | (1 of 20) PF01822 - WSC domain (WSC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGATGGGGGAGGTGGCAGTGGCGAAGGA |
| Internal bar code: | ATGGGTGACGAAAGGGAATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 16 |
| LEAP-Seq percent confirming: | 61.777 |
| LEAP-Seq n confirming: | 1474 |
| LEAP-Seq n nonconfirming: | 912 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGAGCCATAGCTGCGTG |
| Suggested primer 2: | ACCACAGCGAGAAGCTCATT |