| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091150 |
| Chromosome: | chromosome 13 |
| Location: | 5145678 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607650 | POC15 | Proteome of centriole protein 15; (1 of 1) PTHR10891//PTHR10891:SF5 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGCTCGCGCGGCTCCAGCTTGCCGTTGC |
| Internal bar code: | GTGTACGCTTGTATTAAATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 327 |
| LEAP-Seq percent confirming: | 99.6696 |
| LEAP-Seq n confirming: | 3922 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACAACGGACTCACCCTC |
| Suggested primer 2: | CAACATCGGTGACAAGTTGG |