| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091153 |
| Chromosome: | chromosome 10 |
| Location: | 6224161 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464500 | PDO1 | Protein disulfide oxidoreductase; (1 of 3) PTHR13887//PTHR13887:SF10 - GLUTATHIONE S-TRANSFERASE KAPPA // FRNE PROTEIN-LIKE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGAAGGTAGCCCCCTGGTATTGCTGGG |
| Internal bar code: | CTGGGGCACGGAGCGGAAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 69.4624 |
| LEAP-Seq n confirming: | 969 |
| LEAP-Seq n nonconfirming: | 426 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCCGAAGTTGGAAGCTG |
| Suggested primer 2: | TTGCTACCGCTGTTTCCTCT |