Insertion junction: LMJ.RY0402.091173_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.091173
Chromosome:chromosome 10
Location:1447537
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g428550 DTS1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGACCAACATGGTGAGCCTGGAGGACCC
Internal bar code:AGGTATGGCCGCCCATTCATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:153
LEAP-Seq percent confirming:99.9112
LEAP-Seq n confirming:1125
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTCGAGACAGGGTCTTCT
Suggested primer 2:GTAGCTCATAGCTGTCCGCC