Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.091178 |
Chromosome: | chromosome 6 |
Location: | 194192 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g250500 | (1 of 2) 4.2.1.93 - ATP-dependent NAD(P)H-hydrate dehydratase / ATP-dependent H(4)NAD(P)OH dehydratase | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTCGGAGGTCCGCCGGATTGTGCCCGC |
Internal bar code: | TGGGCGGGCCATGTGGACTGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 437 |
LEAP-Seq percent confirming: | 98.3796 |
LEAP-Seq n confirming: | 425 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGGAGGGGAATAATAAGGG |
Suggested primer 2: | CCTGACAAGTCATGAAGGCA |