| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091190 |
| Chromosome: | chromosome_4 |
| Location: | 381914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g217914 | FAP57 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGACTCGCAAGAAAGAGAGGCGTGTTAAC |
| Internal bar code: | CGAGGGGCCTTAGCAATTCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 392 |
| LEAP-Seq percent confirming: | 99.4464 |
| LEAP-Seq n confirming: | 4132 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCATGAATGTAGCCCCC |
| Suggested primer 2: | CACACGCGAGAGGTCTGATA |