| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091203 |
| Chromosome: | chromosome 16 |
| Location: | 81394 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695700 | (1 of 12) PF04749 - PLAC8 family (PLAC8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAAAGTCCAGCCAGTCTTGACAATTAG |
| Internal bar code: | GAGCATCAACCGACTCGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 637 |
| LEAP-Seq percent confirming: | 99.6071 |
| LEAP-Seq n confirming: | 507 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGAGGCCGTACTTCTTGC |
| Suggested primer 2: | GACCACGGTCAGTCGTTTTT |