| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091216 |
| Chromosome: | chromosome 12 |
| Location: | 2371638 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507400 | LCS3,CGL105 | (1 of 4) K01897 - long-chain acyl-CoA synthetase (ACSL, fadD); Long-chain acyl-CoA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGAGTGGGTTCTGCGCAGCCTGGTCGC |
| Internal bar code: | TATGCGACCAGACCGGGACTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 98.725 |
| LEAP-Seq n confirming: | 5188 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTAAGGGGGAGAGGAACG |
| Suggested primer 2: | CCAGTCGCTCCTTCACTAGG |