| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091219 |
| Chromosome: | chromosome 17 |
| Location: | 266193 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698100 | DeSI-3,DESI3 | (1 of 6) PF05903 - PPPDE putative peptidase domain (Peptidase_C97); DeSI-type SUMO protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGATCCAAACACCGAACAAACGCCAGCG |
| Internal bar code: | AGTGAGGCCGAGCAGCAGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 76 |
| LEAP-Seq percent confirming: | 99.1489 |
| LEAP-Seq n confirming: | 8271 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCTTGGGACAGGAGAGT |
| Suggested primer 2: | CCTCAGGAGGAGAGCACATC |