| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091222 |
| Chromosome: | chromosome 9 |
| Location: | 7487674 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413850 | GT90-17,GT90F17 | GT90 family protein 17; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCGTCCTTGTGCTTCTTCATGATGGGG |
| Internal bar code: | GAAGAAGCAATAACTACGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 99.0026 |
| LEAP-Seq n confirming: | 2680 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGTATAGCGAACTGCG |
| Suggested primer 2: | CTGCATGACGCCTCTTTACA |