| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091232 |
| Chromosome: | chromosome 2 |
| Location: | 1901971 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g087700 | APX1 | (1 of 1) PTHR31356//PTHR31356:SF1 - FAMILY NOT NAMED // L-ASCORBATE PEROXIDASE S, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Ascorbate peroxidase 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCTCATGCGCCAGCTCCTTAGCCTTTC |
| Internal bar code: | CTACAAACCCGATAACGGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 767 |
| LEAP-Seq percent confirming: | 99.0554 |
| LEAP-Seq n confirming: | 3041 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCCATGCGGTAGAACAC |
| Suggested primer 2: | CGGTGGCAACTTAAGGGTTA |