| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091268 |
| Chromosome: | chromosome 2 |
| Location: | 8697913 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142186 | FTSZ2 | Plastid division protein FtsZ2 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATCTCCCAGCATCCTAGCTTTTAACCGT |
| Internal bar code: | CCGATATCGGGAGGGTTGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 51 |
| LEAP-Seq percent confirming: | 94.2308 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAAACTCCCCCAAAACT |
| Suggested primer 2: | GTTTACTCGAAGTGCCGCTC |