| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091282 |
| Chromosome: | chromosome 14 |
| Location: | 3684896 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631650 | FAP406 | Flagellar Associated protein 406 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTGCGAGCGGCAGCTGGAGTGGCGTG |
| Internal bar code: | GTGCTTAGGTAAACAAACCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 551 |
| LEAP-Seq percent confirming: | 98.8557 |
| LEAP-Seq n confirming: | 2419 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGGTCTGTGCCTTGCTA |
| Suggested primer 2: | CCATATAACCCACGGTTTCG |