| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091293 |
| Chromosome: | chromosome 15 |
| Location: | 308932 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635700 | MAPKKK12 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED; Mitogen-Activated Protein Kinase Kinase Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATATGCTAGTAGTGCGCTTGTCCGGATA |
| Internal bar code: | GAATGTCTGTCCGAGGTTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 453 |
| LEAP-Seq percent confirming: | 87.1749 |
| LEAP-Seq n confirming: | 9319 |
| LEAP-Seq n nonconfirming: | 1371 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTCTGTACACCCTCTGGA |
| Suggested primer 2: | GCTTGGCTACTGCCTTTCAC |