| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091294 |
| Chromosome: | chromosome 2 |
| Location: | 641368 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077976 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCGTGCGCACAGCTGCACGGAGCTGCT |
| Internal bar code: | TATTCGTAGAGTAGCTTCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.6912 |
| LEAP-Seq n confirming: | 3228 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCACCAGATGGGTATCA |
| Suggested primer 2: | GGCAAGATACCGACCCTGTA |