Insertion junction: LMJ.RY0402.091312_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.091312
Chromosome:chromosome 7
Location:3504277
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g335950 (1 of 2) K10523 - speckle-type POZ protein (SPOP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATACGAGCACCGCAGTCTGCCGGTTCAGC
Internal bar code:CCCGATGAGATTGGGCAATATA

Confirmation - LEAP-Seq

LEAP-Seq distance:612
LEAP-Seq percent confirming:88.5438
LEAP-Seq n confirming:1283
LEAP-Seq n nonconfirming:166
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGTTTTGTTTGTGCGTG
Suggested primer 2:ATTTGGGAGATGAAGCATCG