| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091322 |
| Chromosome: | chromosome 13 |
| Location: | 601254 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g565675 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCAAGGATATGTCCAGCGGCGTCACGG |
| Internal bar code: | GTTGGACTAGATAGTGGACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 99.5994 |
| LEAP-Seq n confirming: | 8204 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTACTGATTGAGGCCAT |
| Suggested primer 2: | GTGACATCAGCTCAGCAGGA |