Insertion junction: LMJ.RY0402.091401_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.091401
Chromosome:chromosome 11
Location:491744
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g467597 (1 of 1) IPR000104//IPR003439//IPR003593//IPR013525//IPR013581//IPR027417 - Antifreeze protein, type I // ABC transporter-like // AAA+ ATPase domain // ABC-2 type transporter // Plant PDR ABC transporter associated // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATCGAACTAGGACGGTCGCGGCCCTCAG
Internal bar code:GACTAAAACGGTGTTCCTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:175
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCGTACATCTGCTCAACC
Suggested primer 2:AGCCCAAGTAGGGAGGGTAA