| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091404 |
| Chromosome: | chromosome 5 |
| Location: | 1147248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245701 | FAP104 | Flagellar Associated Protein 104 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCACCTGCCCCCCGCCTGCCCTTCCA |
| Internal bar code: | TCTCGCGGATTTCTCAGGAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 99.0223 |
| LEAP-Seq n confirming: | 1418 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTCAGAGTTGAAACAGC |
| Suggested primer 2: | CTGCATACCTGGAACCCAGT |