| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091414 |
| Chromosome: | chromosome 2 |
| Location: | 5630740 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109366 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGCGGCGTGTGCCTGTTGCACGGCCCCG |
| Internal bar code: | TGGGTAGTGGTCCAGTGCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 93.0238 |
| LEAP-Seq n confirming: | 4267 |
| LEAP-Seq n nonconfirming: | 320 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAATAGACCCGCAACTCG |
| Suggested primer 2: | GACCCTATCCGACGTTGAAA |