| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091474 |
| Chromosome: | chromosome 2 |
| Location: | 991074 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080250 | YGG1,CGL97,YLMG1 | Conserved in the green lineage 97; (1 of 4) PF02325 - YGGT family (YGGT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCATGCCGCAGTCAAGGCCGCAGTCCC |
| Internal bar code: | CGAGGAAGTATCGTCTGAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 195 |
| LEAP-Seq percent confirming: | 84.7853 |
| LEAP-Seq n confirming: | 691 |
| LEAP-Seq n nonconfirming: | 124 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTAAGAGTCGTGGGGAG |
| Suggested primer 2: | TAGCACATGATGTCGGTGGT |