Insertion junction: LMJ.RY0402.091481_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.091481
Chromosome:chromosome 7
Location:1588471
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCGCGGACTCAGCTGCACACGTTTAG
Internal bar code:CTCTTCTTGCCTTACAAGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:487
LEAP-Seq percent confirming:97.2477
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACGATCGCCGTTTTAAT
Suggested primer 2:GTGCTACTCGGGTGTTTGGT