| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091492 |
| Chromosome: | chromosome 1 |
| Location: | 6685333 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048000 | TPM1 | Tropomyosin 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGATGCTGATGACTGCATGCAGCGTG |
| Internal bar code: | GCGATGAGTCCATGGTGACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 384 |
| LEAP-Seq percent confirming: | 98.6328 |
| LEAP-Seq n confirming: | 505 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGCGTTACCGCATATTT |
| Suggested primer 2: | AGGCAATGTCAAAGGTGTCC |