| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.091494 |
| Chromosome: | chromosome 8 |
| Location: | 1969116 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g367800 | NDH2 | Nucleoside diphosphate hydrolase; (1 of 1) 3.6.1.13//3.6.1.22 - ADP-ribose diphosphatase / ADPR-PPase // NAD(+) diphosphatase / NADP pyrophosphatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGTCGAGTGATGCCAATCGATGGAGTCG |
| Internal bar code: | TGCAGGGGGGACTAGCTAGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1192 |
| LEAP-Seq percent confirming: | 99.3819 |
| LEAP-Seq n confirming: | 4663 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAAGAGATCCGTGTGCAG |
| Suggested primer 2: | GGCTGATGATCTTGCCATCT |