Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.091498 |
Chromosome: | chromosome_17 |
Location: | 5944476 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g740261 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGGTGAGGCAGGGCGGCGGGCTGGAGGTG |
Internal bar code: | CAACTGCTCTCGCACAGTGGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 326 |
LEAP-Seq percent confirming: | 99.7219 |
LEAP-Seq n confirming: | 10039 |
LEAP-Seq n nonconfirming: | 28 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCTTCAGTAACCCCCGTAT |
Suggested primer 2: | GTTCAGTGTGGTGTCGGATG |