Insertion junction: LMJ.RY0402.091513_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.091513
Chromosome:chromosome 4
Location:3496139
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g227950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGAAACAATCGGGGTGAATGGGGGTTGG
Internal bar code:GAGAGCTGCAAGTGACACGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:172
LEAP-Seq percent confirming:99.3475
LEAP-Seq n confirming:609
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCGGTCTAGAGTCAGCC
Suggested primer 2:AGTGGGTTAAGGGTGCTGTG