| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091542 |
| Chromosome: | chromosome 16 |
| Location: | 5121928 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683900 | GDPD2,GDP2 | (1 of 3) K01126 - glycerophosphoryl diester phosphodiesterase (E3.1.4.46, glpQ, ugpQ); Glycerophosphoryl diester phosphodiesterase family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCATGCAGAGCGGTGGCGGTCATGAGCA |
| Internal bar code: | TCTCCTTGGGGGGGATACATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 291 |
| LEAP-Seq percent confirming: | 59.0092 |
| LEAP-Seq n confirming: | 1346 |
| LEAP-Seq n nonconfirming: | 935 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGCTTTAACCACGTTT |
| Suggested primer 2: | CATTGTGCTCGACGTGAGTT |