| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091551 |
| Chromosome: | chromosome 17 |
| Location: | 5592441 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737752 | CDS/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAAAATAGCAATGTCGCTCCAAGCGGTC |
| Internal bar code: | GAACCGTATGCTTGCATTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 98.3673 |
| LEAP-Seq n confirming: | 1446 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGCTCAGTGTCCGTTCA |
| Suggested primer 2: | GGTACATCATCTCGTGCCCT |