| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091556 |
| Chromosome: | chromosome 8 |
| Location: | 932615 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361800 | RPC31 | (1 of 1) PF11705 - DNA-directed RNA polymerase III subunit Rpc31 (RNA_pol_3_Rpc31); DNA-directed RNA polymerase III | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGTGACAGCGGAAAATGGGGGGAAGC |
| Internal bar code: | AAGTACGCGATTATACGCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 151 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCTCCACCCAAGCAAAG |
| Suggested primer 2: | TTTTGTGCAGGAGTCTGACG |