Insertion junction: LMJ.RY0402.091557_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.091557
Chromosome:chromosome 8
Location:740405
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g360500 ERM2 (1 of 1) PF13967//PF14703 - Late exocytosis, associated with Golgi transport (RSN1_TM) // Cytosolic domain of 10TM putative phosphate transporter (PHM7_cyt); ERD4-related membrane protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCTTGGGCATTGACAAGTGCGGAAAGG
Internal bar code:GGAAGTGGCTCGCTCGGGAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:330
LEAP-Seq percent confirming:97.9161
LEAP-Seq n confirming:3571
LEAP-Seq n nonconfirming:76
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAGGTTGTGCACTTGCTG
Suggested primer 2:ACCATGAGCCAGAAAACCAC