| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.091571 |
| Chromosome: | chromosome 10 |
| Location: | 6211378 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464400 | SSA20 | Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCGATGAGCCTGGCCGGTGTAACTGCC |
| Internal bar code: | ACCGAGAATCTGTAGTGGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 88.1766 |
| LEAP-Seq n confirming: | 20576 |
| LEAP-Seq n nonconfirming: | 2759 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTCGTTTCCCAAATCCA |
| Suggested primer 2: | GAACCTTTGCAGCTCAGTCC |