| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.091571 |
| Chromosome: | chromosome 10 |
| Location: | 6211397 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464400 | SSA20 | Predicted protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACAACAACTCACCGTGGCCCAAGAGGGT |
| Internal bar code: | GGGGTCGTCTCCTAGATGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 256 |
| LEAP-Seq percent confirming: | 98.7578 |
| LEAP-Seq n confirming: | 159 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTCGTTTCCCAAATCCA |
| Suggested primer 2: | GAACCTTTGCAGCTCAGTCC |