| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.091573 |
| Chromosome: | chromosome 7 |
| Location: | 2607166 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330150 | (1 of 7) IPR011335 - Restriction endonuclease type II-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAATTGCCCATATGCTGCACATTCGCAT |
| Internal bar code: | TTCCAGTGCAGAACAGCCGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 479 |
| LEAP-Seq percent confirming: | 96.4337 |
| LEAP-Seq n confirming: | 676 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGAACAGAGCAGTCACCA |
| Suggested primer 2: | TTCTCCTCCTCCTCCTCCTC |