Insertion junction: LMJ.RY0402.091659_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.091659
Chromosome:chromosome 10
Location:976623
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC intron|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGCACATGTGCAATCACAACACACACACT
Internal bar code:AGGGGGGAAGGCGTAAGGGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:50
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGATGTTTGGAAAAGCGGTA
Suggested primer 2:GAGAAGGGAGTGGCACTTTG